Journal: Nature communications

Article Title: Biosilicification-mimicking chiral nanostructures for targeted treatment of inflammatory bowel disease.

doi: 10.1038/s41467-025-57890-8

Figure Lengend Snippet: Fig. 4 | Pathogenic factors scavenging properties of PEI-L/D-TA@MON. a Molecular model and electrostatic potential of the pathogenic factors and PEI-L/ D-TA@MON. b MD simulation on the adsorbing of pathogenic factors onto PEI-L/D- TA@MON. cThe EINT and electrostatic force (EEF) of the adsorbing of pathogenic factors onto PEI-L/D-TA@MON. d Equations on the scavenging of various patho- genic factors. Time-dependent (e). cfDNA and (f) LPS scavenging capacities of nanomedicines. Data are presented as the mean ± SD (n = 3 independent experi- ments). ***P < 0.001 by two-tailed Student’s t test. g FTIR and h. XPS spectra of PEI- L-TA@MON before and after incubation with H2O2. Concentration-dependent

Article Snippet: The H2O2 elimination capacity of nanomedicines was assayed by H2O2 Colorimetric Assay Kit (Elabscience, Wuhan).

Techniques: Two Tailed Test, Incubation, Concentration Assay

Journal: Cell & Bioscience

Article Title: Modelling porcine NAFLD by deletion of leptin and defining the role of AMPK in hepatic fibrosis

doi: 10.1186/s13578-023-01124-1

Figure Lengend Snippet: Detection of oxidative stress in LEPTIN −/− pig livers. A Expression analysis of CYP2 family genes by real-time PCR. B. WB analysis and Immunofluorescence staining of CYP2E1. Scale bar represents 100 μm. C – E Detection of oxidative stress markers in the liver and serum. Content of MDA ( C ), NO ( D ) and SOD ( E ). N = 3/group. The bars represent the mean ± SD; *P < 0.05

Article Snippet: The total SOD activity detection kit (WST-8 method) (Beyotime, S-0101), lipid oxidation (MDA) assay kit (Beyotime, S-0131) and nitric oxide detection kit (Beyotime, S-0021) were utilized following the manufacturer’s instructions.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining